Senior Biology Deadly Extended Experimental Investigations. RESOURCES FOR QUEENSLAND STUDENTS TEACHERS DEADLY EEI IDEAS Ideas for Year 1. Biology Extended Experimental Investigations. From Dr Richard Walding, BApp. Sc, MSc, MPhil, Ph. D, FAIP, FRACI, CChem, Griffith University, Australia. Senior Physics teacher Moreton Bay College, Brisbane Senior Biology Chemistry teacher Moreton Bay College 2. Author New Century Senior Physics textbook by Oxford University Press. Email richardwalding. S2215017X15000454-gr5.jpg' alt='How To Prepare Serial Dilutions Practice' title='How To Prepare Serial Dilutions Practice' />Many of the suggestions below involve the use of animals. Various laws apply to the use of animals in schools particularly any live non human vertebrate, that is fish, amphibians, reptiles, birds and mammals, encompassing domestic animals, purpose bred animals, livestock, wildlife, and also cephalopods such as octopus and squid. For some further advice go to Use of Animals note at the end. How To Prepare Serial Dilutions Practice' title='How To Prepare Serial Dilutions Practice' />OPEN INQUIRY EEI In most Queensland schools the Year 1. EEI provides students with a limited range of research questions to investigate, usually drawn from a single unit eg plants. This allows students to focus on the process and techniques of investigation and the subsequent reporting. The examples from Nanango State High and Moreton Bay College in Plant Options below are of this type. However, in Year 1. These Open Inquiry EEIs may provide more ownership, engagement and deeper understanding for the students but do place a bigger demand on resources and laboratory management. However, many teachers have found strategies to manage this should they choose to opt for a Open EEIs. An example of an Open Inquiry EEI can be downloaded here Nanango SHS Year 1. Open EEI. A Materials Requisition Form accompanies this document. My thanks to Biology teachers from Nanango for sharing this. How To Prepare Serial Dilutions Practice' title='How To Prepare Serial Dilutions Practice' />SAFETY Risk Assessment in an EEI School. S have implemented various forms of Risk Assessment for their science laboratory or practical work. Examples of Risk Assessment forms can be found in the tasks available on this page. A comprehensive form developed over several years by Urangan SHS can be found here Risk Assessment Form. It is only an example and should not be assumed to necessarily meet the legal and safety obligations of any particular school or situation. TWO MAIN APPROACHES FOR AN EEI In general there are two main methods used in senior high school EEIs. They are The method of artificial variation where you manipulate one variable to see the effect on the other and keeping the rest constant For example, what is the effect of temperature the manipulated or independent variable, IV on the enzyme digestion of starch the dependent variable, DV. The method of concomitant variation where some naturally occurring variation in some condition Variable 1 is correlated against some other condition Variable 2. This is also called a correlation method. You can think that nature has manipulated the variables but it is still appropriate to class one as dependent and one as independent. David Douillet Judo. For example, do young leaves have the same density and distribution of stomata as older leaves or how does temperature IV in a natural environment affect stoma opening DV In this second case you do not need you to control the environmental temperature, but you do need to measure the DV at different temperatures. The difficulty with the second approach is the control of other potentially influential variables such as humidity as you have to take what you get. However, that does not preclude the variables in relationship being considered the IV and DV or being graphed as such. One way to address the confounding variables eg humidity is to collect data on the other variable as well. That is, call the stomatemperature data Part I, and call the stomahumidity data Part 2. You can run the statistics on each pair separately, but, for students who are not that stats savvy then they could look for interactions between them at a visual level. How To Prepare Serial Dilutions Practice' title='How To Prepare Serial Dilutions Practice' />1. Simple Dilution Dilution Factor Method based on ratios A simple dilution is one in which a unit volume of a liquid material of interest is. Genetics, Bio260 The Ames Test E. De Stasio The Ames test was developed in the 1970s by Bruce Ames, Professor of Biochemistry at UCBerkeley, as a fast and. My thanks to Marilyn Love, Science Department, All Hallows School, Brisbane for this example. FOOD TECHNOLOGY OPTIONSDoes the amount of bacterial growth in food differ according to its preparation or handling Although some micro organisms are deliberately used to make foods such as yoghurt and cheese, other microbes spoil food. Food, as well as meeting the nutritional requirements of humans, will also meet the nutritional needs of a vast range of micro organisms. These microbes will multiply rapidly in food, given the appropriate conditions such as temperature, p. H and moisture. The flavour, aroma and texture will ultimately be affected. Microbial contamination accounted for 3. Food Standards Australia New Zealand FSANZ between 1 January 1. December 2. 00. 4. Of these recalls, 4. Listeria monocytogenes contamination 1. Salmonella contamination and 1. Escherichia coli contamination Food Standards 2. Those most affected by food poisoning are the elderly, the young and immune suppressed individuals. Luigi Mansion Dark Moon. This EEI is suggested by Biology teacher Sylvia Hicks from St Aidans College, Corinda. Effects of different anti microbials on bacterial growth Joseph Lister first introduced aseptic surgery in 1. C6. H5. OH as a germicide. He was able to reduce mortality of post operative surgery by up to 4. Since then the control of growth by antimicrobial compounds has grown into a multi billion dollar industry. A good EEI is to assess the effect of a variety of antimicrobial disinfectants on bacterial growth. Charmaine Keal HOD Science at Tullawong State High School offered this advice the most popular is testing the effects of different anti microbials on bacterial growth. Krypton Toolkit. Our labbies prepare the plates and order in E. Students then put a wash of E. Small paper discs like out of a hole punch are sterilised and then soaked into different anti microbials. The discs are placed onto the agar plates and then into the incubator for a couple of days. If the anti bacterial is effective there is a clear ring around the disc where the E. Students have used the width of this ring to indicate effectiveness and to collect quantitative data. The experiment can work quite well keen students have then researched the active compounds within the antibiotic, how they act upon the bacteria and link to the results observed. Common antibacterials are alcohols eg ethanol, isopropyl alcohol, synthetic detergents QAC quaternary ammonium compounds or phenols. No single disinfectant is ideal. Each has its advantages and disadvantages. For example, phenols sterilise well but are corrosive and toxic. Detergents and 7. At Mt Maria College, Enoggera, Brisbane, the Science Co ordinator Shan Wainwright also uses multi disks impregnated with antibiotics. Risk assessment including disposal of waste is vital. The images in the diagram to the right were prepared by Dr Dennis Kunkel of Dennis Kunkel Microscopy Inc. Over years Dennis has assembled a vast collection of these micrographs, many of which have been colorized and can be viewed on his website. Images Copyright Dennis Kunkel Microscopy, Inc. Some photos below taken from the EEI of a Yr 1. Biology student at Our Ladys College, Annerley, Brisbane.